KMID : 0880220140520080681
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Journal of Microbiology 2014 Volume.52 No. 8 p.681 ~ p.688
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A potent Brucella abortus 2308 ¥Äery live vaccine allows for the differentiation between natural and vaccinated infection
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Zhang Junbo
Yin Shuanghong Guo Fei Meng Ren Chen Chuangfu Zhang Hui Li Zhiqiang Fu Qiang Shi Huijun Hu Shengwei Ni Wei Li Tiansen Zhang Ke
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Abstract
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Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (¥Äery) was constructed to overcome these drawbacks. The growth of the ¥Äery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, ¥Äery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-¥ã) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the ¥Äery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.
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KEYWORD
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Brucella abortus 2308, ery promoter mutant, live attenuated vaccine, EryA
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